HyStem-C is recommended for stem cell researchers as a starting point for optimization of a cell’s microenvironment since it contains Gelin-S. Gelin-S, or thiolated gelatin (denatured collagen), allows attachment of a wide variety of cell types and takes the guesswork out of the appropriate attachment factors to use.
HyStem-C provide an excellent starting point for optimizing the matrix for stem cell culture. Since its composition can be easily tailored to find suitable matrix compositions. Unlike an animal-derived extracellular matrix (ECM), HyStem-C is fully chemically defined. The hydrogels are based on three biocompatible components: thiol-modified hyaluronan (a major constituent of native ECM), thiol-modified gelatin (denatured collagen), and a thiol-reactive crosslinker, polyethylene glycol diacrylate (PEGDA). HyStem-C hydrogels can be customized by adding ECM proteins and by varying the hydrogel compliance to match the stiffness of native tissues.