PhotoGel® UV Methacrylated Gelatin Kit
Three dimensional (3D) gels allow for the study of the effects of the mechanical properties of the extracellular matrix (ECM), such as density and rigidity, on cell development, migration, and morphology. Unlike 2D systems, 3D environments allow cell extensions to simultaneously interact with integrins on all cell surfaces, resulting in the activation of specific signaling pathways.
Gel stiffness or rigidity also affects cell migration differently in 3D versus 2D environments. Furthermore, integrin-independent mechanical interactions resulting from the entanglement of matrix fibrils with cell extensions are possible in 3D systems, but not in 2D systems where the cells are attached to a flat surface.
Advanced BioMatrix offers PhotoGel®, a purified gelatinmethacrylate kit, which provides native-like 3D gelatin gels with the unique attributes to be prepared at various concentrations and UV-crosslinked to provide various gel stiffness. Though it is denatured collagen, gelatin retains many natural binding motifs such as RGD and MMP sites.