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Papain Dissociation System

Short info

For researchers in the field of cell biology, CellSystems offers the full range of products for cell isolation and tissue dissociation. With the Worthington Biochemical Corporation enzymes and biochemicals you will maximize the yield of functionally viable, dissociated cells.

Flyer PDS

Proteolytic enzymes are widely used in cell dissociation

With some tissues papain has proved less damaging and more effective than other proteases. Lam found that of the enzymes used for dissociating turtle retina, papain produced the least trauma. Intact single photoreceptor cells have been isolated from adult salamander retina with papain. Huettner and Baughman described a method using papain to obtain high yields of viable, morphologically intact cortical neurons from postnatal rats. Finkbeiner and Stevens applied the Huettner and Baughman method to the dissociation of postnatal rat hippocampus. Papain is used with fetal as well as postnatal brain regions to provide maximal dissociation and viability of neurons.

The Worthington Papain Dissociation System (PDS) is a set of reagents intended for use in the tissue dissociation method of Huettner and Baughman. The materials are designed for convenience and simplicity and are useful to the occasional user as well as the more experienced and frequent user. Each lot is use tested for performance in tissue dissociation and provides freshly prepared enzyme solutions for each dissociation.*

* Text is quoted from the Worthington Enzyme Manual.

Product Name Code Activity Source
PDS Kit, Inhibitor Vial OI-BSA ≥300 mg TRL inhibited per vial Egg White/BSA
PDS Kit, Papain Vial PAP2 ≥100 units per vial Papaya Latex
Papain Dissociation System PDS
Papain Dissociation System, Without EBSS PDS2