Neutral Protease (Dispase®)
For researchers in the field of cell biology, CellSystems offers the full range of products for cell isolation and tissue dissociation. With the Worthington Biochemical Corporation enzymes and biochemicals you will maximize the yield of functionally viable, dissociated cells.
Neutral Protease (Dispase) is a bacterial enzyme produced by Bacillus polymyxa that hydrolyses N-terminal peptide bonds
of non-polar amino acid residues and is classified as an amino-endopeptidase. Its mild proteolytic action makes the enzyme especially useful for the isolation of primary and secondary (subcultivation) cells since it maintains cell membrane integrity.
Neutral Protease (Dispase) is also frequently used as a secondary enzyme in conjunction with collagenase and/or other proteases in many primary cell isolation and tissue dissociation applications. Neutral Protease (Dispase) dissociates fibroblast-like cells more efficiently than epithelial-like cells so it has also been used for differential isolation and culture applications. Other advantages are its non-mammalian (bacterial) source and its ability to be inhibited by EDTA.*
* Text is quoted from the Worthington Enzyme Manual.
|Neutral Protease (Dispase), Purified||NPRO||≥4 units per mg dry weight||Bacillus polymyxa|