TDGF1

Cat.-Nr.: CS-C1395

Recombinant Human Teratocarcinoma-Derived Growth Factor 1

TDGF1 Human Recombinant produced in HEK-293 cells is a single, glycosylated, polypeptide chain containing 139 amino acids with a molecular weight of 17.8 kDa though on SDS-PAGE migrates at about 25kDa due to the glycosilation. The TDGF1 is purified by proprietary chromatographic techniques.

Specifications PDF

Amino acid sequence

LGHQEFARPS RGYLAFRDDS IWPQEEPAIR PRSSQRVPPM GIQHSKELNR TCCLNGGTCM LGSFCACPPS FYGRNCEHDV RKENCGSVPH DTWLPKKCSL CKCWHGQLRC FPQAFLPGCD GLVMDEHLVA SRTPELPPS.

Source

HEK293-F Cells.

Purity

Greater than 95.0% as determined by SDS-PAGE.

Formulation

The TDGF1 protein was lyophilized from a 0.2µm filtered solution containing no additives or preservatives.

Stability

Lyophilized TDGF1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TDGF1 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Size

10µg

Solubility

It is recommended to reconstitute the lyophilized TDGF1 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Physical appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Biological activity

In a functional ELISA, immobilized recombinant human Activin RIB/Fc chimera receptor (0.2 µg/mL) will bind TDGF1 with linear range of 0.8 to 100 ng/ml.

Endotoxin

Endotoxin tested

Usage

For research use only - not approved for human or veterinary use or for diagnostic or clinical procedures.