PGRN Human

Cat.-Nr.: CS-C1346

Recombinant Human Progranulin

Progranulin Human Recombinant fused to FLAG at C-terminus produced in HEK is a single, glycosylated, polypeptide chain containing 593 amino acids and having a molecular mass of 88 kDa. The Progranulin is purified by standard chromatographic techniques.

Specifications PDF

Source

HEK 293 cells.

Purity

Greater than 90.0% as determined by(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.

Formulation

The protein solution contains 1x PBS.

Stability

Progranulin although stable at 4°C for 3 weeks, should be stored at -20°C for longer periods of time.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).

Size

10µg

Physical appearance

Sterile Filtered clear solution.

Biological activity

1. The effects of phospho-ERK1/2 and non phospho-ERK1/2 by human progranulin-FLAG in THP-1 cells.To examine the signal of phospho-p44/42 MAPK and p44/42 MAP kinase, reactions were carried out at 37 degrees Celsius over 0, 30, 60, minutes, respectively by adding the recombinant protein (100ng/ml) to the THP-1 monocyte cell, which was maintained with serum starvation for 24hrs. Recombinant proteins in lanes 1, 2, and 3 were subjected to THP-1 monocyte cell treatments over 0, 30, 60mins, respectively.2. The effects of phospho-ERK1/2 and non phospho-ERK1/2 by human progranulin-FLAG in MCF10A cells. To examine the signal of phospho-p44/42 MAPK and p44/42 MAP kinase, reactions were carried out at 37 degrees Celsius over 0, 10, 30, 60mins, respectively by adding the recombinant protein(500ng/ml) to the MCF10A human breast epithelial cells, which was maintained with serum starvation for 24hrs. Recombinant proteins in lanes 2, 3, and 4 were subjected to MCF10A human breast epithelial cell treatments over 0, 10, 30, 60mins, respectively.

Endotoxin

Endotoxin tested

Usage

For research use only - not approved for human or veterinary use or for diagnostic or clinical procedures.