IL 6 Human, CHO
Recombinant Human Interleukin-6, CHO
Interleukin-6 Human Recombinant produced in CHO is a single, glycosylated polypeptide chain containing 185 amino acids and migrates at 22 kDa as a glycosilated protein on SDS-PAGE.The IL6 is purified by proprietary chromatographic techniques.
Amino acid sequence
APVPPGEDSK DVAAPHRQPL TSSERIDKQI RYILDGISAL RKETCNKSNM CESSKEALAE NNLNLPKMAE KDGCFQSGFN EETCLVKIIT GLLEFEVYLE YLQNRFESSE EQARAVQMST KVLIQFLQKK AKNLDAITTP DPTTNASLLT KLQAQNQWLQ DMTTHLILRS FKEFLQSSLR ALRQM.
Chinese Hamster Ovarian Cells.
Greater than 97.0% as determined by(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
Lyophilized from a solution containing Phosphate- Buffered Saline pH-7.4.
Lyophilized Interleukin-6 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL6 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
It is recommended to reconstitute the lyophilized Interleukin-6 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Sterile Filtered White lyophilized (freeze-dried) powder.
The activity was measured in a cell prolifiration assay using B9 cells. The ED50 for this effect is 8-20 pg/ml.
For research use only - not approved for human or veterinary use or for diagnostic or clinical procedures.