FGF 1 Human
Recombinant Human Fibroblast Growth Factor-acidic
Fibroblast Growth Factor-acidic Human Recombinant (FGF-1) produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 141amino acids and having a molecular mass of 15967 Dalton.The FGF acidic is purified by proprietary chromatographic techniques.
Amino acid sequence
MFNLPPGNYK KPKLLYCSNG GHFLRILPDG TVDGTRDRSD QHIQLQLSAE SVGEVYIKST ETGQYLAMDT DGLLYGSQTP NEECLFLERL EENHYNTYIS KKHAEKNWFV GLKKNGSCKR GPRTHYGQKA ILFLPLPVSS D.
Greater than 97.0% as determined by(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
The protein was lyophilized from a concentrated (1mg/ml) sterile solution containing 10mM sodium phosphate pH=7.5 and 75mM NaCl.
Lyophilized Fibroblast Growth Factor-1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution FGF-a should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles.
It is recommended to reconstitute the lyophilized Fibroblast Growth Factor-acidic in sterile 18MΩ-cm H2O at 4 degrees Celsius at a concentration of 0.1mg-0.25mg per 1ml. Allow sample to sit for 5 min. at 4 degrees, spin to remove precipitant.
Sterile Filtered White lyophilized (freeze-dried) powder.
The ED50, calculated by the dose-dependant proliferation of mouse BALB/c 3T3 cells is
For research use only - not approved for human or veterinary use or for diagnostic or clinical procedures.