Products » Reference Standards - Molecular, Cell, and Histological

Reference Standards - Molecular, Cell, and Histological

Our Isogenic cell line-based FFPE reference standards represent ideal materials for diagnostic assay development and routine quality control purposes. ONCOREF™ FFPE Mutated Cell Scrolls provides precision-edited, isogenically-paired, mutant and wild-type FFPE reference standards is a great resource for diagnostic assay development and quality control purposes.  ONCOREF™ footprint-free genome editing technology for cell line generation enable precise mutation generation without concerns of genomic scar incorporation from the use of selection markers or other footprint-based technologies.
 

Why Use Cell and Molecular Reference Materials?

  • Reference materials for biologics  provide a consistent and reliable resource for evaluating and optimizing various stages of your sample processing workflow.  Whether you’re starting from DNA extraction, assay design, or library preparation, our reference materials can help you to identify and eliminate sources of variability within your protocols. Our reference standards for biologics is ideal for companion diagnostic assay development. 
  • To learn more about the ONCOREF™ products

Molecular Reference Materials Enables  You  To:

  • Optimize your DNA extraction protocols prior to using valuable patient samples
  • Monitor the impact of workflow changes on downstream results using consistent, third-party validated standards
  • Establish limits of detection (LODs) using mixtures of isogenically-paired, mutant and wild-type DNA
  • Utilize standardized materials to easily transfer workflows across labs or institutions
  • Accurately evaluate batch-to-batch variability to streamline your quality control process
  • Our reference standards for biologics are ideal for companion diagnostic assay development. 

Applications:

Our cellular or molecular reference materials can be used for various histological or genetic analysis methodologies.

  • Immunohistochemistry (IHC)
  • H&E staining 
  • in situ hybridization (ISH & FISH)
  • DNA sequencing (Sanger, Next-Generation Sequencing)
  • PCR (quantitiative real-time or digital)
  • DNA microarray  
  • RNA (gene-expression)
  • QDP

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  • Genomic DNA (gDNA)

    The ONCOREF™ Genomic DNA (gDNA) Reference Standards represent biologically-relevant control material that can be used for diagnostic assay development or can be used in routine monitoring of assay performance in a CLIA laboratory environment.  The ONCOREF™ Genomic DNA (gDNA) Reference Standards can be directly incorporated into the sample processing workflows for assay optimization, evaluate assay sensitivity and specificity, and analyze the impact of workflow changes on downstream analysis. 

  • Frozen Cell Pellet

    Derived from our engineered isogenic cell line, the ONCOREF™ Frozen Cell Pellet is a biologically-relevant reference material that can be used to test formalin-fixation's effect on nucleic acid integrety in the presence of celluar matrix.  

  • Custom FFPE Service

    Our Custom FFPE Service uses a patent-pending process to generate highly homogenous FFPE blocks containing any desired cell density (up to 90% area coverage). Both morphology and biological molecules, including protein and nucleic acid (DNA and RNA) targets are well preserved for IHC and ISH analysis.

  • FFPE Mutation Cell Blocks

    The ONCOREF™ FFPE Mutation Cell Blocks provide a reliable and renewable source of clinically relevant specimens for assay development or for routine clinical laboratory quality control (QC) use.

  • FFPE Mutation Cell Scrolls

    Our ONCOREF™ FFPE Mutation Cell Scrolls use 20 µm sections which are rolled into scrolls or (rolls) and placed in a nucelic acid-free tube for easy and quick nucleic acid extraction. 

  • FFPE Mutation Cell Slides

    The ONCOREF™ FFPE Mutation Cell Slides are perfect controls for ISH or IHC analysis.  Cells are harvested by a proprietary trypsin-free method to best preserve cell surface antigens while inflicting minimal physical damage that leads to loss of biomarkers. Nucleic acids (DNA/RNA) are perfectly preserved in the 5 µm sections.  

  • CELL-REF™ FFPE Human Cancer Cell Slides

    We offers slide-mounted FFPE cancer tissue derived cell line sections slides from 34 common cancer cell lines, derived from 10 different tissues of origin.  Our FFPE cell pellets are prepared using a patent-pending process that generates highly homogenous FFPE blocks with any cell density (up to 90% area coverage). Both morphology and biological molecules, including protein and nucleic acid (DNA and RNA) targets are well preserved for IHC and ISH analysis.

  • TISSU-REF™ FFPE Mouse Tissue Slides

    The TISSU-REF™  FFPE Mouse Tissue Slides used a patent-pending process to prepare highly homogenous FFPE sections with consitant and equallly distributed cell density.  Both morphology and biological molecules, including protein and nucleic acid (DNA and RNA) targets are well preserved for IHC and ISH analysis.