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Induced Pluripotent Stem Cells (iPSCs)

Applied StemCell, Inc. provides Induced Pluripotent Stem (iPS) cells at low passages (p12). These pluripotent cells were generated from normal human skin fibroblast using a non-integrating, footprint-free reprogramming method (1). This method allows the transient expression of human transcription factors (OCT4, SOX2, KLF4, and c-MYC) that initiate the reprogramming process (2). The resulting human iPS cells (hiPSCs) were selected using morphological criteria without the use of either fluorescent markers or drug selection. These iPS cells have been tested for the expression of the pluripotency markers, including OCT4, SOX2, SSEA4, and TRA-1-60, AP (alkaline phosphatase) activity and for functional pluripotency via teratoma formation analysis. Detailed protocols for thawing, culturing under both feeder and feeder-free conditions, and cryopreservation of these iPS cells are provided.

 

Available iPSCs are:

- iPS Cells (iPSC from Fibroblasts, Episomal, Male, Control Lines for Genome Editing Services)

- Induced Pluripotent Stem Cells (Control Lines from CD34+ Blood Cells;Female)

 

In addition, further iPSCs (healthy and diseased) are available.

 

Please contact us for further information.