Hepatocyte Isolation System
For researchers in the field of cell biology, CellSystems offers the full range of products for cell isolation and tissue dissociation. With the Worthington Biochemical Corporation enzymes and biochemicals you will maximize the yield of functionally viable, dissociated cells.
Most traditional methods published for isolating hepatocytes use crude and partially purified enzyme preparations
including various types of collagenase and other proteases. More recently the use of better characterized preparations of collagenase such as Worthington Types 1 and 4 (CLS-1, 4) have provided better results. All crude collagenase preparations can contain lot-variable contaminating proteases, esterases and other enzymes requiring researchers to pre-screen several lots of enzyme and/or continually modify isolation parameters and protocols.
The Worthington Hepatocyte Isolation System (HIS) has been developed to provide researchers with a reliable, convenient, and consistent hepatocyte cell isolation system. By using the pre-optimized combination of enzymes contained in this kit, it is possible to minimize the lot-to-lot variation and improve the quality of the isolated hepatocytes. In addition, Worthington use-tests each lot by isolating hepatocytes from adult rat to assure performance, reliability, and consistent yield of viable cells.
The method is based on that described by Berry, M.N., modified by Seglen, P.O. (Methods in Cell Biology, vol XIII, David M. Prescott ed., Academic Press, 1976; Chapter 4, "Preparation of Isolated Rat Liver Cells", pp 29-83), and further optimized in conjunction with several researchers.*
* Text is quoted from the Worthington Enzyme Manual.
|DNase Vial (D2)||D2||≥1,000 units per vial||Bovine Pancreas|
|Hank's Balanced Salt Solution 10X (HBSS10)||HBSS10|
|Hepatocyte Isolation System||HIS|
|0.15M, MOPS Buffer, (MOPS)||MOPS|
|Sodium Bicarbonate, 7.5%, (NAH)||NAH|
|L-15 Media Powder (L15NK)||L15NK|